Page 10 - أبحاث كلية الصيدلة 2016-2020

2016 Publications

International Journal of Pharmacy and Pharmaceutical Sciences

ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF LECTIN
PROTEIN CONTENTS IN CHAMERION ANGUSTIFOLIUM L. DRIED RAW

MATERIAL AND THE STUDY OF ITS ACTIVITY USING
RATUSERYTROAGGLUTINATION

ZEAD HELMI MAHMOUD ABUDAYEH, KHALDUN MOHAMMAD AL AZZAM, ULIANA
VLADIMIROVNA KARPIUK, LOAY KHALED HASSOUNEH, AMJAD ABUIRMEILEH

Objective: To isolate, identify and quantify lectin protein contents in Chamerion
angustifolium L. dried raw material species namely; leaves in the flowering stage, buds,
and flowers and the study of its activity.
Methods: Lectin activity has been determined using the biological method called
ratuserytroagglutination. This method is based on the formation of aggregates of lectins
and rat erythrocytes using the floor amount of lectins that agglutinate erythrocytes as a
unit of measurement. Additionally, the protein contents of the extracts have been
determined using the Bradford assay method.
Results: lectins activity from Chamerion angustifolium L. in leaves in the flowering
stage, buds, and flowers were 2.72±0.06, 0.24±0.008, and 0.56±0.014 units/mg protein,
respectively. The greatest lectins activity was in the leaves in the flowering stage
followed by flowers and then in buds. Protein contents in leaves in the flowering stage,
buds, and flowers were 4.71±0.03, 6.77±0.02, and 5.76±0.14 mg/ml, respectively.
Conclusion: All proteins obtained from the Chamerion angustifolium L. plant raw
material were shown to possess rat erythrocytes agglutinating activity. The crude
extract of leaves in the flowering stage exhibited the strongest hemagglutinating activity
of about 2.72 units/mg proteins, whereas the buds showed the lowest activity of about
0.24 units/mg proteins. It should be highlighted herein, although many plant lectins
mimic the behavior of plant storage proteins, these lectins should not be classified as
storage proteins.

https://innovareacademics.in/journals/index.php/ijpps/article/view/9678

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